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You are here:Home » Biochemicals » Biochem-Maleimide, MTS & Sulfhydryl » Sulfo-N-succinimidyl3-[[2-(p-azidosalicylamido)ethyl]-1,3’-dithio]propionate (SASD)

Sulfo-N-succinimidyl3-[[2-(p-azidosalicylamido)ethyl]-1,3’-dithio]propionate (SASD)

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Specifications

A photo-reactive heterobifunctional cross-linker with hydroxyphenyl azide and Sulfo-NHS ester reactive groups. Can be used to perform iodination reactions.
Catalog #S8020-25
Spacer Arm 18.9 Angstroms
Solubility DMSO, Hot Water
Storage and HandlingSome methanethiosulfonates are hygroscopic and all hydrolyze in water, over a period of time, particularly in the presence of nucleophiles. They should be stored in a desiccator at minus 20°C and warmed up to room temperature before opening the vial. For maximum results, solutions should be made up immediately prior to use even though solutions in distilled water appear to be stable for hours at 4°C.
At pH 7.5 and room temperature, MTSEA hydrolyzes with a half-life of about 15 minutes, MTSET hydrolyzes with a half-life of 10 minutes, and MTSES hydrolyzes with a half-life of 20 minutes. Routinely, one can use 2.5mM MTSEA, 1mM MTSET, or 10mM MTSES, applied for 1 to 5 minutes. (MTSET is 2.5 times as reactive with small sulfhydryl compounds as is MTSEA, and 10 times as reactive as MTSES). (Stauffer and Karlin, 1994; Karlin, personal communication).
For application to Xenopus Oocytes, dissolve the compounds in 115mM NaCl, 2.5mM KCl, 1.8mM MgCl2, 10mM Hepes, pH 7.5 with NaOH. MTS reagents decompose in buffer very quickly.
•DMSO is a good solvent for the MTS reagents which are not water soluble (i.e. the non-charged MTS reagents).
Molecular FormulaC16H16N5NaO9S3
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
Alternate namesSASD


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