Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Superoxide Dismutase » Anti -Superoxide Dismutase, Cu,Zn (SOD Cu,Zn)

Anti -Superoxide Dismutase, Cu,Zn (SOD Cu,Zn)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Superoxide dismutase (SOD) is an endogenously produced
intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2¯ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16kD each are linked by two cysteines forming an intra-subunit disulfide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80kD. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30kD and it exists only in the extracellular space (9). SOD3 can also be distinguished by its heparin-binding capacity (1).
Applications:
Suitable for use in Western Blot, Immunoprecipition, Immunohistochemistry and ELISA. Other applications not tested.
Catalog #S8060-09B
Recommended DilutionWestern Blot (Colorimetric): 0.2ug/ml
Immunoprecipitation: 10ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.0, 0.1% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman Cu/Zn SOD
SpecificityRecognizes human Cu/Zn superoxide dismutase (SOD) at ~23kD. Species crosseactivity: monkey, mouse, rat, bovine, coral, canine, hamster, porcine, rabbit, sheep and Xenopus at ~19kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links