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You are here:Home » Antibodies » Abs to Superoxide Dismutase » Anti -Superoxide Dismutase Cu,Zn (SOD)

Anti -Superoxide Dismutase Cu,Zn (SOD)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Catalog #S8060-09C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:5000. Detects Cu/Zn SOD in RIPA lysates from A431 cells.
Optimal dilutions to be determined by the researcher.
Positive Antigen ControlA0001-50 Non-stimulated A431 cell lysate. Add 2.5ul of 2- mercaptoethanol per 100ul of lysate and boil for 5 minutes to reduce the preparation. Use 10ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50%) glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride containing 0.05% sodium azide.
PuritySerum
ImmunogenRecombinant protein corresponding to full-length human SOD (superoxide dismutase) plus an N-terminal methionyl.
SpecificityRecognizes Cu/Zn SOD, Mr 16kD. Species Crossreactivity: Human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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