Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Superoxide Dismutase » Anti -Superoxide Dismutase 3 (Superoxide Dismutase 3 Extracellular, SOD 3, SOD3, Extracellular Superoxide Dismutase [Cu Zn], Extracellular Superoxide Dismutase Precursor, EC SOD, MGC20077)

Anti -Superoxide Dismutase 3 (Superoxide Dismutase 3 Extracellular, SOD 3, SOD3,
Extracellular Superoxide Dismutase [Cu Zn], Extracellular Superoxide Dismutase Precursor,
EC SOD, MGC20077)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2 to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16kD each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80kD. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30kD and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).
Catalog #S8060-10N
ApplicationsSuitable for use in Western Blot, Immunohistochemistryand Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot:1:1000
Immunohistochemistry: 1:100
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1K
Clone No10F772
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH7.4, 0.09% sodium azide and 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman EC-SOD purified from aortas.
SpecificityRecognizes EC-SOD. Species Crossreactivity: rat, other species not tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links