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You are here:Home » Antibodies » Abs to Superoxide Dismutase » Anti -Superoxide Dismutase, Cu,Zn (SOD1)

Anti -Superoxide Dismutase, Cu,Zn (SOD1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Catalog #S8060-14E
Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. There are three major families of superoxide dismutase, depending on the metal cofactor Cu-Zn (which binds both copper and zinc), Fe and Mn types (which bind either iron or manganese), and finally the Ni type, which binds nickel.
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Paraffin): Does not require pre-treatment (trypsin or pronase) prior to staining or antigen retrieval using heat treatment methods prior to staining of paraffin sections.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Positive ControlHuman spleen and lymph node.
HybridomaSp-2/0-Ag14 myeloma cell line with spleen cells from immunized mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10F478
HostMouse
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSuperoxide dismutase enzyme prepared from bovine erythrocytes.
SpecificityRecognizes bovine Superoxide Dismutase, Cu, Zn. Does not cross react with immunoglobulins but does react weakly with Mn SOD. Species Crossreactivity: human and mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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