| There are four main surfactant proteins, known as SP-A, B, C, and D. SP-A was the first surfactant protein to be identified, and is also the most abundant. Its molecular mass varies from 26-38kD. It can be found in an open or closed form depending on the other substances present in the system. Calcium ions produce the closed-bouquet form. SP-A is necessary for the production of tubular myelin, a lipid transport structure unique to the lungs. |
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| Catalog # | S8400 |
| Applications | Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested. |
| Recommended Dilutions | Immunohistochemistry: 1:4000-1:8000 with and without antigen retrieval. See Protocol. |
| Western Blot: 1:500. Detected glycosylated and unglycosylated isoforms of SP-A on 10ug of rat lung lysates; Band at ~26kD. |
| ELISA: 1:500-1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb |
| I1904-43: IgG, X-Adsorbed (Biotin) Pab Gt x Rb |
| I1904-40E: IgG, H&L (Biotin) Pab Gt x Rb |
| I1904-46P: IgG, H&L (Biotin) Pab Gt x Rb |
| I1904-42T: IgG, H&L (Biotin) Pab Gt x Rb |
| I1904-42V: IgG, H&L (Biotin) Pab Gt x Rb |
| Recommended Control | Lung tissue, lung lavage |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid in PBS. No preservative added. |
| Purity | Serum |
| Immunogen | Human SP-A (deglycosylated, beta-eliminated) purified from human alveolar proteinosis material. |
| Specificity | Reacts strongly with both glycosylated and unglycosylated isoforms of human SP-A at 26kD. Immunostains alveolar Type II cells, macrophages, and alveolar proteinosis material in both human proteinosis and in animal models of alveolar proteinosis. Species Crossreactivity: primate, rat and mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
