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You are here:Home » Antibodies » Abs to Proteins » Anti -Surfactant Protein B (Precursor)

Anti -Surfactant Protein B (Precursor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IF
Pulmonary surfactant is a complex mixture of phospholipids and proteins that is secreted from type II cells in alveoli and reduces the surface tension at the alveolar air-liquid interface, providing alveolar stability necessary for normal ventillation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C, and D. SP-A (28-36kD) and SP-D (43kD) are collagenous carbohydrate-binding proteins, whereas SP-B (8-9kD) and SP-C (4kD) are noncollagenous hydrophobic proteins. SP-B is expressed in pulmonary adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of the lung and nonpulmonary adenocarcinomas do not express SP-B. ProSP-B (42-46kD) is glycosylated in the Golgi apparatus and undergoes carboxy-and aminoterminal proteolysis by a cathepsin D-like protease.
Catalog #S8401-35
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive controlLung adenocarcinomas.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No3F338
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See S8401-35X
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant pro-surfactant protein B. Cellular Localization: Cytoplasmic.
SpecificityRecognizes human pro-SP-B at ~42-46kD. Does not recognize mature SP-B. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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