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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH IF

Pulmonary surfactant is a complex mixture of phospholipids and proteins that is secreted from type II cells in alveoli and reduces the surface tension at the alveolar air-liquid interface, providing alveolar stability necessary for normal ventillation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C, and D. SP-A (28-36kD) and SP-D (43kD) are collagenous carbohydrate-binding proteins, whereas SP-B (8-9kD) and SP-C (4kD) are noncollagenous hydrophobic proteins. SP-B is expressed in pulmonary adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of the lung and nonpulmonary adenocarcinomas do not express SP-B. ProSP-B (42-46kD) is glycosylated in the Golgi apparatus and undergoes carboxy-and aminoterminal proteolysis by a cathepsin D-like protease.
Catalog #	S8401-35
Applications	Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
	Optimal dilutions to be determined by the researcher.
Positive control	Lung adenocarcinomas.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	3F338
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See S8401-35X
Purity	Purified by Protein A affinity chromatography.
Immunogen	Recombinant pro-surfactant protein B. Cellular Localization: Cytoplasmic.
Specificity	Recognizes human pro-SP-B at ~42-46kD. Does not recognize mature SP-B. Species Crossreactivity: rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.