Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Proteins » Anti -Surfactant Protein D, Lectin Domain (SP-D)

Anti -Surfactant Protein D, Lectin Domain (SP-D)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Surfactant Protein D is a Ca2+ dependent carbohydrate binding protein with a structure similar to other C-type mammalian lectins. Its molecular weight is 620kD under non-reducing conditions and 43kD under reducing conditions. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A. SP-D is a lung surfactant associated collectin that has been implicated in the innate pulmonary host defense against a wide variety of important bacterial, fungal and viral pathogens.
Catalog #S8402
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (Use snap frozen or paraffin embedded tissue): Fresh rat tissue: 1:400; fresh human tissue: 1:20. Fixation: Acetone 5 minutes at 4°C, paraformaldehyde, paraffin. Tissue pretreatment: Microwave treatment 2 x 5 minutes at 750W for paraffin tissues.
ELISA: Use as a capture antibody.
Optimal dilutions to be determined by the researcher.
Source Tissue culture supernatant
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q2261
HostMouse
SourceRat
Concentration~0.4mg/ml
FormSupplied as a lyophilized powder from buffer, 5mg/ml BSA, 0.09% sodium azide. Reconstitute with 500ul sterile ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified rat surfactant protein D from bronchoalveolar lavage.
SpecificityRecognizes an epitope localized in the lectin domain of rat surfactant protein D (SP-D). Reacts with alveolar type II cells, alveolar macrophages, types of Clara cells SP-A+ and SP-A- and extracellular deposits. Does not react with Alveolar type III cells. No reaction on rat skin, colon , kidney or liver. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links