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Anti -Surfactant Protein D, Lectin Domain (SP-D)
Anti -Surfactant Protein D, Lectin Domain (SP-D)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH |
|
| Surfactant Protein D is a Ca2+ dependent carbohydrate binding protein with a structure similar to other C-type mammalian lectins. Its molecular weight is 620kD under non-reducing conditions and 43kD under reducing conditions. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A. SP-D is a lung surfactant associated collectin that has been implicated in the innate pulmonary host defense against a wide variety of important bacterial, fungal and viral pathogens. | | | Catalog # | S8402 | | Applications | Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested. | | Recommended Dilutions | Immunohistochemistry (Use snap frozen or paraffin embedded tissue): Fresh rat tissue: 1:400; fresh human tissue: 1:20. Fixation: Acetone 5 minutes at 4°C, paraformaldehyde, paraffin. Tissue pretreatment: Microwave treatment 2 x 5 minutes at 750W for paraffin tissues. | | ELISA: Use as a capture antibody. | | Optimal dilutions to be determined by the researcher. | | Source | Tissue culture supernatant | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 2Q2261 | | Host | Mouse | | Source | Rat | | Concentration | ~0.4mg/ml | | Form | Supplied as a lyophilized powder from buffer, 5mg/ml BSA, 0.09% sodium azide. Reconstitute with 500ul sterile ddH2O. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Purified rat surfactant protein D from bronchoalveolar lavage. | | Specificity | Recognizes an epitope localized in the lectin domain of rat surfactant protein D (SP-D). Reacts with alveolar type II cells, alveolar macrophages, types of Clara cells SP-A+ and SP-A- and extracellular deposits. Does not react with Alveolar type III cells. No reaction on rat skin, colon , kidney or liver. Species Crossreactivity: human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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