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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH

Syk, a protein tyrosine kinase (PTK) is expressed in immature T cells, all B cells, basophils, hepatocytes, platelets and erythrocytes. It functions in some T cell receptor signaling processes in addition to B cell receptor and integrin-mediated signal transduction processes. Syk-/syk- mice exhibit defective phagocytosis by macrophages.
Catalog #	S9100-10
Applications	Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistolochemistry (Formalin/paraffin): 1:100 for 30 min. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by the researcher.
Positive Control	Raji cells, Lymphoma
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.6, 0.2% BSA, 15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A synthetic peptide derived from aa313-339 of human Syk protein
Cellular Localization	Cytoplasmic.
Specificity	Recognizes Syk signaling protein, Mr 72kD. The epitope is located at aa313-339. Species Crossreactivity: Human. Does not react with mouse or porcine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.