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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IH IC IF

Synaptophysin (SypI), also referred as p38 (1), constitutes approximately 7% of the total vesicle protein and is selectively associated with small synaptic vesicles (SSVs)
Syp I contains four transmembrane regions and can be phosphorylated on its cytoplasmic C-terminus by both serine/threonine and tyrosine kinases (2). In addition to syp I, synaptic vesicles contain a highly homologous membrane protein named synaptoporin or synaptophysin II (Syp II) (3). While Syp I is ubiquitously expressed and present in virtually all synapses whereas Syp II is expressed at high levels only in selected neurons (4).
Catalog #	S9108-03A
Applications	Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions	Western Blot: 1:10,000 (34kD)
	Immunohistochemistry: 1:250-500
	Immunocytochemistry: 1:250
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	6k388
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50mM Tris-glycine, pH7.4, 0.15M NaCl, 0.01% sodium azide, 0.05% BA and 40% glycerol.
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues in C-terminus (cytoplasmic domain) of human Synaptophysin. MW: 34kD
Specificity	Recognizes human Synaptophysin. Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.