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You are here:Home » Antibodies » Abs to Synaptophysin » Anti -Synaptophysin

Anti -Synaptophysin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH
Synaptophysin labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. Anti-synaptophysin reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas. ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas. Of the epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.
Catalog #S9108-05A
ApplicationsSuitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Paraffin): 1:25. Requires heat induced antigen retrieval using citrate buffer pH 6.0.
Western Blot: 1:500
Optimal working dilutions to be determined by researcher.
Recommended Positive Control TissuePancreas
HybridomaNSO Myeloma cells with B cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM
Clone No3H2894
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide
PurityPurified
ImmunogenCrude human synaptic immunoprecipitate
SpecificityRecognizes human synaptophysin, also known as Major synaptic vesicle protein p38.. Species Crossreactivity: hamster, rat and marmoset
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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