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You are here:Home » Antibodies » Abs to Synaptophysin » Anti -Synaptophysin

Anti -Synaptophysin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH
Synaptophysin labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. Anti-synaptophysin reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas. ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas. Of the epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.
Catalog #S9108-05A
Recommended Secondary Reagents F(ab')2 rabbit anti-mouse IgG HRP
Fusion Partners Spleen cells from immunized BALB/c mice were fused with cells of the NSO mouse myeloma cell line.
Recommended Positive Control Tissue Brain
ApplicationsImmunohistochemistry: Paraffin. 1:500-1:1000
Western Blotting: 1:500
ELISA
Optimal working dilutions to be determined by researcher.
Immunohistochemistry This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Clone TypeMonoclonal
IsotypeIgM
Clone No3H2894
HostMouse
SourceHuman
Concentration~1mg/ml
FormPurified IgM - liquid in PBS with 0.1% sodium azide.
PurityPurified by gel filtration.
ImmunogenCrude human synaptic immunoprecipitate.
Specificity3H2894 recognizes an antigen which appears to be identical to the human synaptic vesicle protein synaptophysin. It reacts with synaptophysin expressed in CHO cells (3., 8.). Species Crossreactivity: 3H2894 reacts with pig, hamster and rat synaptophysin
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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