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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B IH

Synaptophysin is an ~38kD integral membrane protein involved in neurotransmitter exocytosis present on synaptic vesicles. This protein consists of four transmembrane domains, with its amino- and carboxyl-terminus facing the cytoplasm. Due to its transmembrane domain structure, synaptophysin has been postulated to be a gap junction-like protein because voltage-dependent channel activity is seen when synaptophysin hexamers are reconstituted into lipid membranes. Recent studies have shown synaptophysin to be a major cholesterol-binding protein in brain synaptic vesicles and it has been proposed that this cholesterol-binding ability may be of key importance in the generation of synaptic vesicles at plasma membranes. A regional reduction of synaptophysin was found in patients with Alzheimer’s disease and schizophrenia.
Catalog #	S9108-16
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	Western Blot (Colorimetric): 1:2000
	Immunohistochemistry: 1:50 using human neuroblastoma tissue
	Optimal dilutions to be determined by researcher.
	Positive Controls:
	Mouse and rat brain tissue extract
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	4E206
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Purity	Purified by ammonium sulfate precipitation
Immunogen	Synaptic vesicle-containing fractions immunoprecipitated from
human brain homogenates.
Specificity	Recognizes human synaptophysin at ~38kD.
Species Crossreactivity	Mouse, rat and bovine. No reactivity was detected in mouse liver extracts.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.