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You are here:Home » Antibodies » Abs to Synaptophysin » Anti -Synaptophysin

Anti -Synaptophysin

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant E B IP IH IC FC
Synaptophysin is an integral membrane protein of small synaptic vesicles in brain and endocrine cells(1). Synaptophysin (syp I) is a synaptic vesicle membrane protein that constitutes approximately 7% of the total vesicle protein. Multiple lines of evidence implicate syp I in a
number of nerve terminal functions (2). Synaptophysin is one of the most abundant membrane proteins of small synaptic vesicles. In mature nerve terminals it forms a complex with the vesicular membrane protein synaptobrevin, which appears to modulate synaptobrevin's interaction with the plasma membrane-associated proteins syntaxin and SNAP25 to form the SNARE complex as a prerequisite for membrane fusion. It has been shown that synaptobrevin is
preferentially cleaved by tetanus toxin while bound to synaptophysin or when existing as a homodimer (3). Data suggest that synaptophysin is released from the Golgi apparatus in a vesicular form, after glycosylation, and is then transported to nerve endings by a mechanism which requires integrity of microtubules (4).
Applications:
Suitable for use in: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications have not been tested.
Recommended Dilution:
Western Blot: 1:200,000
Immunohistochemistry: 1:250-500
Immunocytochemistry: 1:250-500
Flow Cytometry: 1:20
Immunoprecipitation: 1:50
Optimal dilutions to be determined by researcher.
Catalog #S9108-20
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No8k9
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 0.01% sodium
azide, 0.05% BSA and 40% Glycerol.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues in human Synaptophysin.
SpecificityRecognizes human Synaptophysin. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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