| Synaptophysin is an integral membrane protein of small synaptic vesicles in brain and endocrine cells(1). Synaptophysin (syp I) is a synaptic vesicle membrane protein that constitutes approximately 7% of the total vesicle protein. Multiple lines of evidence implicate syp I in a |
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| number of nerve terminal functions (2). Synaptophysin is one of the most abundant membrane proteins of small synaptic vesicles. In mature nerve terminals it forms a complex with the vesicular membrane protein synaptobrevin, which appears to modulate synaptobrevin's interaction with the plasma membrane-associated proteins syntaxin and SNAP25 to form the SNARE complex as a prerequisite for membrane fusion. It has been shown that synaptobrevin is |
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| preferentially cleaved by tetanus toxin while bound to synaptophysin or when existing as a homodimer (3). Data suggest that synaptophysin is released from the Golgi apparatus in a vesicular form, after glycosylation, and is then transported to nerve endings by a mechanism which requires integrity of microtubules (4). |
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| Applications: |
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| Suitable for use in: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications have not been tested. |
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| Recommended Dilution: |
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| Western Blot: 1:200,000 |
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| Immunohistochemistry: 1:250-500 |
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| Immunocytochemistry: 1:250-500 |
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| Flow Cytometry: 1:20 |
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| Immunoprecipitation: 1:50 |
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| Optimal dilutions to be determined by researcher. |
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| Catalog # | S9108-20 |
| Storage and Stability | |
| May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 8k9 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 0.01% sodium |
| azide, 0.05% BSA and 40% Glycerol. |
| Purity | Supernatant |
| Immunogen | A synthetic peptide corresponding to residues in human Synaptophysin. |
| Specificity | Recognizes human Synaptophysin. Species Crossreactivity: mouse and rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
