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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified E IH

Syndecans are cell surface heparan sulfate proteoglycans that act as co-receptors for growth factor binding, cell-matrix interactions, and cell-cell interactions to regulate cell adhesion, proliferation, and differentiation. Syndecan-2 interacts with matrix proteins such as laminin and fibronectin, and its cytoplasmic V region controls matrix assembly at the cell surface. Syndecan-2 is essential for angiogenic sprouting during embryogenesis and controls the growth of normal human osteoblastic cells. Syndecan-2 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-2 expression has been detected in several different tumor types.
Catalog #	S9111-40B1
Applications	Suitable for use in Direct ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA (Direct): 0.5-1ug/ml. Detection limit for rhSyndecan-2 is ~10ng/well.
	Immunohistochemistry: 25ug/ml Detects Syndecan-2 in paraffin-embedded normal human skin tissue sections.
	Optimal dilutions to be determined by the researcher.
Hybridoma	NSO myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	7H78
Host	Rat
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	NS0-derived recombinant human Syndecan-2 (aa 19-144; Accession # P34741)
Specificity	Recognizes human Syndecan-2. In direct ELISAs, does not crossreact with recombinant mouse Syndecan-1, recombinant human Syndecan-3 or recombinant human Syndecan-4.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.