| Syntaxin is a 35kD type II integral membrane protein localized to the plasma membrane at nerve terminals (2). Syntaxin was identified through its association with synaptotagmin and N-type calcium channel, a subtype of calcium channel on the plasma membrane important for neurotransmitter release. Homologs of syntaxin were identified in various eukaryotes ranging from yeast to human (1, 2). Evidences for syntaxinís involvement in vesicular transport and neurotransmission include the requirement of its yeast homologs, SSO 1 and 2, in targeting and fusion of Golgi-derived vesicles with the plasma membrane (3). Syntaxin is also the target of clostridial neurotoxin C1 that block neurotransmitter release (4). The formation of 20S complex involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF (5). |
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| Catalog # | S9205-01 |
| Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot (Colorimetric) (1,6): 1.0ug/ml |
| Immunohistochemistry: 1:2000 |
| Immunoprecipitation: 1:500 |
| Optimal dilutions to be determined by the researcher. |
| PC-Personal Communication |
| Positive Controls | Rat Brain Tissue Extract |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 4E209 |
| Host | Mouse |
| Source | Human |
| Concentration | As reported |
| Form | Supplied as a liquid in PBS 0.1mM PMSF, 50% glycerol. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Mice were immunized with synaptic vesicle-containing fractions that |
| were immunoprecipitated from human brain homogenates using anti-human synaptophysin monoclonal antibodies |
| Specificity | This antibody detects an ~38kD protein doublet, corresponding to the apparent molecular mass of syntaxin on SDS-PAGE immunoblots, in samples from human, mouse, rat, porcine, feline, bovine, hamster and Xenopus. No reactivity was seen with a 35kDa protein in mouse liver extracts. Some reactivity with higher molecular weight bands of unknown identity is observed on immunoblots. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
