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You are here:Home » Antibodies » Abs to Syntaxin, Synaptotagmin, Synaptophysin » Anti -Syntaxin 1A (Synaptotagmin-Associated 35kD Protein, Neuron-Specific Antigen HPC-1)

Anti -Syntaxin 1A (Synaptotagmin-Associated 35kD Protein, Neuron-Specific Antigen HPC-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Syntaxin 1, also known as HPC-1, is a 35kD integral membrane protein which along with SNAP-25, and VAMP/synaptobrevin plays a role in vesicular trafficking and membrane fusion. Two syntaxin 1 isoforms have been identified, syntaxin 1A which is localized to nerve terminals of sensory neurons and nerve fibers reaching small blood vessels, and syntaxin 1B which is localized to motor end plates and muscle spindles (2). The SNARE (soluble N-ethylmaleimide sensitive fusion protein [NSF] attachment protein [SNAPs] receptors) hypothesis of membrane fusion proposes that syntaxin 1A and SNAP-25 (target membrane SNAREs, t-SNAREs) and VAMP/synaptobrevin (vesicular SNAREs, v-SNAREs) bind together to form a tripartite structure that along with soluble cytosolic proteins allows for close membrane apposition of donor and target membranes thereby facilitating membrane fusion. The interaction of syntaxin 1A with v-SNAREs is thought to be negatively regulated by the binding of munc-18 to syntaxin 1A and this interaction is controlled by Cdk5 phosphorylation of munc-18 (3). Syntaxin 1A can be phosphorylated by casein kinase II and phosphorylation of syntaxin enhances its interaction with synaptotagmin (4).
Applications:
Suitable for use in Western Blot. Other applications not tested.
Catalog #S9205-03E
Recommended DilutionWestern Blot (Colorimetric): 1ug/ml
Optimal dilutions to be determined by the researcher.
Positive Controls:
Mouse Brain Tissue Extract
Rat Brain Tissue Extract
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide derived from rat syntaxin 1A (KLH).
SpecificityRecognizes rat Syntaxin 1A at ~35-36kD.
Species CrossreactivityMouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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