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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

STX5 (Syntaxin 5) is a predicted 41-42kD member of the syntaxin family of proteins. It is a t-SNARE that is widely expressed (but not in striated muscle), and embedded in either the cis-Golgi network (a 35kD form) or the ER (the 42kD form). STX5 is involved in the regeneration and maintenance of the Golgi apparatus after mitosis. Human STX5 is a type IV single-pass transmembrane protein (very long cytoplasmic N-terminus) that is 355aa in length. It contains an ER retrieval signal (Arg4Lys5Arg6), a t-SNARE domain (aa263-325) with a coiled-coil region (aa 287-318), and a C-terminal transmembrane sequence. The 35kD short form exhibits an
alternate start site at Met55. Two other potential splice variants show either the Met55, or a Met97 start site coupled to a 19aa substitution for aa 303-355. Over aa55-300, human STX5 shares 94% aa identity with mouse STX5.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Catalog #	S9205-15
Recommended Dilution	Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well.
	Western Blot: 1ug/ml. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Recombinant corresponding to aa55-300 of human Syntaxin 5 isoform 2 expressed in E. coli (Q13190).
Specificity	Recognizes human Syntaxin 5. Shows < 1% cross-reactivity with rhSyntaxin 7, rhSyntaxin 8 and rhSytaxin 12. Species Sequence Homolgy: Mouse 94%.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.