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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH IF

STX5 (Syntaxin 5) is a predicted 41-42kD member of the syntaxin family of proteins. It is a t-SNARE that is widely expressed (but not in striated muscle), and embedded in either the cis-Golgi network (a 35kD form) or the ER (the 42kD form). STX5 is involved in the regeneration and maintenance of the Golgi apparatus after mitosis. Human STX5 is a type IV single-pass transmembrane protein (very long cytoplasmic N-terminus) that is 355aa in length. It contains an ER retrieval signal (Arg4Lys5Arg6), a t-SNARE domain (aa263-325) with a coiled-coil region (aa 287-318), and a C-terminal transmembrane sequence. The 35kD short form exhibits an alternate start site at Met55. Two other potential splice variants show either the Met55, or a Met97 start site coupled to a 19aa substitution for aa 303-355.
Catalog #	S9205-15B
Applications	Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunofluorescence: 10ug/ml
	Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	5A6
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Recombinant corresponding to aa1-101 from human STX5A with GST tag. MW of the GST tag alone is 26kD.
Specificity	Recognizes human STX5A.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.