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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified E B

STX6 (Syntaxin 6) is a 30-35kD member of the syntaxin family of proteins. It is widely expressed, embedded in trans-Golgi and endosomal membranes, and is associated with a variety of SNARE proteins. STX6 is involved in the regulation of caveolae-dependent endocytosis, and is crucial for membrane remodeling. Human STX6 is a type IV single-pass transmembrane protein (very long cytoplasmic N-terminus) that is 255 amino acids (aa) in length. It contains a coiled-coil region (aa 41-74), a t-SNARE domain (aa 163-225) that is likely involved in protein-protein interactions, and a C-terminal transmembrane sequence. There are two splice variants that are also 255 aa in length, but show variation over aa 69-73. Over aa 69-217, human STX6 shares 93% aa identity with mouse STX6.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Catalog #	S9205-25C
Recommended Dilution	ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Syntaxin 6
	Western Blot: 1ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute in PBS containing 0.02% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Purified, E. coli-derived, recombinant human Syntaxin 6 (rhSyntaxin 6; aa 69-217; Accession# O43752).
Specificity	Recognizes human Syntaxin 6.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.