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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B IH

Alpha-synuclein (SNCA), with 140-amino acid protein (about 15,000 Da), belongs to the synuclein family, which also includes beta- and gamma-synuclein. SNCA is a soluble protein, expressed principally in the brain but also expressed in low concentrations in all tissues examined (except liver). SNCA is implicated in the regulation of dopamine release and transport. The triplication of the SNCA can cause Parkinson disease (PD) and diffuse Lewy body disease within the same kindred. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Immunohistochemistry for SNCA has become the histological technique of choice for the diagnosis for Parkinson's disease, Dementia with Lewy bodies and Multiple System Atrophy.
Catalog #	S9500-01D
Application	Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilution	Western Blot: 1:500-1:2000
	Immunohistochemistry: 1:500-1:2000
	ELISA: 1:10,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Clone No	8A253
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
Purity	Purified by crude ascites
Immunogen	Ni-NTA purified truncated recombinant SNCA expressed in E. coli strain BL21 (DE3)
Specificity	Recognizes truncated SNCA recombinant protein (WB), human SNCA recombinant protein (ELISA), human glioma tissue (IHC).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.