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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH

Synnuclein family of proteins is abundantly expressed in neuronal cytosol and presynaptic terminals. In vertebrates they are encoded by three different genes. Synucleins have been specifically implemented in three major diseases: Alzheimer’s (AD), Parkinson’s (PD) and breast cancer. In AD, a peptide derived from alpha-synuclein forms an intrinsic component of plaque amyloid. In PD, an alpha-synuclein accumulates in Lewy bodies. An allele of alpha-synuclein has been linked to many familial cases of PD. In breast cancer increased expression of gamma synuclein correlates with the disease progression. Synucleins appear to be involved in the membrane plasticity in developing song control system of songbirds.
Catalog #	S9500-11
Applications	Suitable for use in Western Blot, Immnoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (formalin/paraffin): 1:200 for 30 min at RT
	Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by the researcher.
Positive Control	Brain
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3F344
Host	Mouse
Source	Human
Concentration	0.1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide before the addition of glycerol to 40%. Also available without BSA and azide. See S9500-11X
Purity	Purified by Protein G affinity chromatography.
Immunogen	Human recombinant alpha-synuclein.
Cellular Localization	Neuronal cytosol and presynaptic terminals
MW of Antigen	~14kD
Specificity	Recognizes Human recombinant alpha-synuclein.
Species Crossreactivity	Highly specific for alpha-synuclein and does not react with other synuclein subtypes. Does not react with mouse or rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.