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S9500-21C Rabbit Anti-Synuclein, alpha (SNCA, 127750, P37840, MGC110988, NACP, PARK1, PARK4, non-A beta Component of AD Amyloid, NACP, Parkinson Disease Familial 1, PD1)

Specifications
References
Clone Type
Monoclonal
Host
Rabbit
Source
Mouse
Swiss Prot
O55042
Isotype
IgG
Clone Number
10E50(D37A6)
Grade
Purified
Applications
IF IHC IP WB
Crossreactivity
Mo Rt
Gene ID
20617
Shipping Temp
Blue Ice
Storage Temp
-20°C

a-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. a-synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Mutations of the a-synuclein gene have been linked to Parkinson disease.

Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochmistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 Requires citrite HIER. Immunofluorescence (IF-F): 1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide surrounding Glu105 of mouse a-synuclein protein.
Form
Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recognizes endogenous levels of mouse alpha Synuclein at 18kD. |Species Crossreactivity: rat
References
1. Goldberg, M.S. and Lansbury Jr., P.T. (2000) Nat. Cell Biol. 2, 115-119.
USBio References
No references available
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