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You are here:Home » Antibodies » Abs to Synuclein » Anti -Synuclein, alpha, beta, nitrated

Anti -Synuclein, alpha, beta, nitrated

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IH IC
Catalog #S9500-28A
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot 1:5,000-1:10,000 detects nitrated a- or b-Synuclein recombinant protein. Nitrated and unnitrated a-synuclein recombinant protein were resolved by electrophoresis, transferred to nitrocellulose and probed with antinitro-a/b-Synuclein (1:5,000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. nitrated a- Synuclein (~14.5kD). Note. The detected band at ~30kD represents the formation of o,o’ –dityrosine cross-links occurring during nitration with peroxynitrite (5)
Immunohisto/cytochemistry Detects nitrated-synuclein in human tissues (1)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No4H376
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid with 0.05% sodium azide.
PurityAscites
ImmunogenFull-length nitrated recombinant human a-synuclein.
SpecificityRecognizes recombinant, nitrated a-synuclein, Mr 14.5kD or nitrated b-Synuclein, Mr 17kD. Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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