Synuclein-beta is a 19kD, 134aa cytoplasmic phosphoprotein that is a member of a family of small, highly conserved proteins, including synucleins alpha, beta, and gamma (synoretin), localized predominantly in presynaptic nerve terminals in the brain. Synuclein-beta associates with Synuclein-alpha, preventing it from aggregating. Abnormal Synuclein-alpha aggregates form filamentous inclusions (Lewy bodies) in neurodegenerative diseases including Parkinson's disease. Within the region used as an immunogen, human SNCB shares 99% aa identity with mouse and rat Synuclein-beta.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 2ug/ml using human hippocampal tissue Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human brain (medulla and caudate putamen) Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Gly47-Glu120 from human Synuclein-beta, expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human Synuclein-beta. Does not crossreact with recombinant human Synuclein-alpha in ELISA.