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You are here:Home » Antibodies » Abs to Binding Proteins » Anti -Synaptopodin (SYNPO, KIAA1029)

Anti -Synaptopodin (SYNPO, KIAA1029)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74kD, pI 9.27. In SDS-PAGE the antigen appears as 100kD polypeptide in brain and 110kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).
Catalog #S9500-74
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (frozen sections and paraffin-embedded tissue after microwave treatment): 1:5-1:10. Incubate for 1hr at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F198
HostMouse
SourceRat
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenIsolated rat kidney glomeruli.
SpecificityRecognizes rat synaptopodin at ~44kD (degradation product). Species Crossreactivity: human, bovine, guinea pig, mouse, and gerbil. Does not recognize rabbit, frog or chicken. Recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells); co-localizes with a-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression in cultured hippocampal neurons and during postnatal maturation of rat brain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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