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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IH IF

Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74kD, pI 9.27. In SDS-PAGE the antigen appears as 100kD polypeptide in brain and 110kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).
Catalog #	S9500-74A
Applications	Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (Frozen): Neat
	Immunohistochemistry (Paraffin): Requires microwave treatment prior to incubation.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	9L671
Host	Mouse
Concentration	Not Determined
Form	Supplied as a liquid, 0.01% sodium azide.
Purity	Supernatant
Immunogen	Isolated rat kidney glomeruli.
Specificity	Recognizes rat synaptopodn. Reacts with differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation- dependent expression during postnatal maturation of rat brain. Differentiation- dependent expression in cultured hippocampal neurons. Species Crossreactivity: human, bovine, mouse, guinea pig and gerbil. Does not react with rabbit, frog, or chicken.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.