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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH IC FC

The synucleins (there is an alpha, beta, and gamma isoform) are small proteins, less than 140 amino acids, which may function in mictotubule assembly and organization. Synucleins with nitrated tyrosine residues are found in the hallmark lesions of Parkinson's and Lewy Body disease. It has been proposed that the formation of aggregates of nitrated proteins may be at least partly responsible for the cytoxicity of these neurodegenerative diseases.
Catalog #	S9502-04D
Applications	Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:20
	Western Blot: 1:2000
	Immunohistochemistry (paraffin): 1:100-250
	Immunocytochemistry: 1:50-100
	Immunoprecipitation: 1:40
	Optimal dilutions to be determined by the researcher.
Positive Control	Fetal brain lysate
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9i220
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Purity	Supernatant
Immunogen	Synthetic peptide corresponding to residues on Synuclein, gamma.
Specificity	Recognizes human Synuclein, gamma. Species Crossreactivity: rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.