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You are here:Home » Antibodies » Abs to Synuclein » Anti -Synuclein, gamma CT (BCSG1, SYN)

Anti -Synuclein, gamma CT (BCSG1, SYN)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43aa peptides (beta amyloid 1-40, 1-42 and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140aa a-synucleins is identical with non-Ab component (NACP) of AD. The 134aa b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14. Mutations in a-synuclein gene causing a replacement of alanine with a threonine has the potential to cause the protein to misfold. Synucleins are primarily expressed in the brain. At least 3 forms, two large (140aa SYN-1 & 149aa SYN-2) and a small form (SYN-3, 42aa) are produced by alternative splicing. Recently a new isoform, termed g-synuclein (SNCG) or breast cancer gene 1 (BCG1) has been cloned (human 127aa, rat/mouse 123aa). All three synucleins shown ~40% identity. g-synuclein is primarily expressed in brain (substantia nigra), and over expressed in advanced infiltrating carcinoma of breast, and in ovarian cancer.
Catalog #S9502-10A
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Western Blot: 1-10 ug/ml using Chemiluminescence technique
Optimal dilutions to be determined by the researcher.
Control PeptideS9502-10P: Synuclein, gamma CT Control Peptide (BCSG1, SYN)
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder in 100mM Tris, pH 7.5, 0.2% BSA, 0.1% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide17aa near the C-terminus of human g-synuclein (KLH coupled).
SpecificityRecognizes human gamma-synuclein. No significant sequence homology with alpha- or beta-synucleins, or other proteins. Species Sequence Homology: No significant sequence homology is seen with either rat/mouse gamma-synuclein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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