| Catalog # | S9505-01 |
| Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors. The family includes three known proteins | alpha-synuclein, beta-synuclein, and gamma-synuclein. The alpha- and beta-synuclein proteins are found primarily in brain tissue, where they are seen mainly in presynaptic terminals. The gamma-synuclein protein is found primarily in the peripheral nervous system and retina, but its expression in breast tumors is a marker for tumor progression. |
| Applications | Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 2-4ug/ml for 2 hours at RT (reported). |
| Immunohistochemistry (Formalin/paraffin): 1:200 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Brain |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide, before the addition of glycerol to 40%. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A synthetic peptide derived from near N-terminal of human alpha, beta and gamma synuclein proteins. |
| Cellular Localization | Neuronal cytosol and presynaptic terminals. |
| Specificity | Recognizes human pan synuclein. Species Crossreactivity: Rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
