| 5'-A C G T^-3' |
|
| 3'-^T G C A-5' |
|
| Tail-neoschizomer of MaeII, produces DNA fragments that have a 4-base 3’-extension |
|
| Catalog # | T0716 |
| Concentration | ~10u/ul |
| Source | Thermus aquaticus Cc1–331 |
| Restriction Enzyme Buffer E, 10X; R1625-04, Included | 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 65°C** |
| Diluent Buffer | Dilute with 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. |
| Storage Buffer | Supplied as a liquid in 10mM Tris-HCl (pH 7.5 at 25°C), 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. |
| Overdigestion Assay | |
| No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with TaiI. |
|
| Ligation/Recutting Assay | |
| After 50-fold overdigestion (3 units/ug DNA x 17 hours) with TaiI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 1.5uM. More than 95% of these can be recut. |
|
| Labeled Oligonucleotide (LO) Assay | |
| No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. |
|
| Methylation Effects | |
| TaiI does not cut Am5CGT. Blocked by CG methylation. |
|
| Stability during Prolonged Incubation | |
| A minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 65°C. |
|
| Thermal Inactivation | |
| Enzyme is not inactivated by incubation at 80°C for 20min. |
|
| Compatible Ends | |
| AatII |
|
| Number of Recognition Sites in DNA | |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
