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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » TaiI (MaeII)

TaiI (MaeII)


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5'-A C G T^-3'
3'-^T G C A-5'
Tail-neoschizomer of MaeII, produces DNA fragments that have a 4-base 3-extension
Catalog #T0716
SourceThermus aquaticus Cc1331
Restriction Enzyme Buffer E, 10X; R1625-04, Included 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 65°C**
Diluent BufferDilute with 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage BufferSupplied as a liquid in 10mM Tris-HCl (pH 7.5 at 25°C), 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with TaiI.
Ligation/Recutting Assay
After 50-fold overdigestion (3 units/ug DNA x 17 hours) with TaiI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 1.5uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Methylation Effects
TaiI does not cut Am5CGT. Blocked by CG methylation.
Stability during Prolonged Incubation
A minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 65°C.
Thermal Inactivation
Enzyme is not inactivated by incubation at 80°C for 20min.
Compatible Ends
Number of Recognition Sites in DNA
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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