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You are here:Home » Antibodies » Abs to Signal Transduction » Anti -Talin (KIAA1027, TLN, TLN1, ILWEQ)

Anti -Talin (KIAA1027, TLN, TLN1, ILWEQ)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IP IH IC
Catalog #T0750
ApplicationsSuitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects Talin in a human A431 RIPA cell lysate. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with T0750 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunocytochemistry/Immunohistochemistry 10ug/ml of a previous lot showed positive immunostaining for Talin in Ref-52 cells fixed with 4% paraformaldhyde and permea-bilized with 0.25% Triton X-100. A previous lot of this antibody detected talin in frozen sections.
Immunoprecipitation 4ug detects Talin from a human A431 RIPA lysates.
Positive Control IncludedA0001-50: Non-stimulated A431 cell lysate. Use 20ug per lane for minigels.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.T.146
HostMouse
SourceHuman
Concentration1mg/ml
FormSupplied as liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by Ion-exchange chromatography.
ImmunogenHuman Talin.
SpecificityRecognizes human Talin at ~ 200kD. Species crossreactivity: Rabbit and chicken. Does not cross-react with mouse or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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