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You are here:Home » Antibodies » Abs to Signal Transduction » Anti -Talin (KIAA1027, TLN, TLN1, ILWEQ)

Anti -Talin (KIAA1027, TLN, TLN1, ILWEQ)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
Immunoblots the 225kD talin band on SDS gels of platelets and other cells. It may be used to stain adhesion plaques in fixed and permeabilised fibroblasts. T0750-20A recognizes an epitope within residues 139-433. This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.
Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Catalog #T0750-20A
Recommended DilutionsFlow Cytometry: 1:25-1:100; 10ul labels 10e6 cells in 100ul. Membrane permeabilisation is required.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
HybridomaSpleen cells from immunized BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
Positive ControlMost tissues
Recommended Negative ControlI1904-78R: IgG1 Murine Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
Clone TypeMonoclonal
IsotypeIgG1
Clone No9i120
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman platelet talin
SpecificityRecognizes an epitope within residues 139-433 of human talin at ~225kD. Stains adhesion plaques in fixed and permeabilized fibroblasts. Species Crossreactivity: rabbit, chicken
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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