Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Tamm-Horsfall Glycoprotein (Uromucoid, THgp, THP, Uromodulin, UMOD)

Anti -Tamm-Horsfall Glycoprotein (Uromucoid, THgp, THP, Uromodulin, UMOD)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Sheep Purified E IH
Tamm-Horsfall glycoprotein (THP), which is synthesized by renal tubular cells, is the most abundant protein in normal human urine. Although its physiological function remains unclear, it has been proposed that THP may act as a defence factor against urinary tract infections by inhibiting the binding of S- and P-fimbriated Escherichia coli to renal epithelial cells. Because THP-related proteins are also found in the superficial layers of the oral mucosa, THP may interfere with the cytoadherence of pathogenic bacteria that colonize mucosal surfaces other than those of the urogenital tract. It has been shown that THP binds to virulent Bordetella pertussis and reduces its adherence to both renal and pulmonary epithelial cells. This cytoadherence inhibitory effect was not observed with a B. pertussis mutant lacking the pertussis toxin (PTX) operon, and was dependent on the direct interaction of THP with the S2 subunit within the PTX B oligomer. It has also been shown that the glycosylation moiety of THP is crucial for its binding to PTX. The THP-PTX interaction was exploited to develop an affinity chromatography method that allows a one-step purification of active PTX. These observations suggest that besides its anti-adherence activity, THP may also trap toxins produced by pathogenic bacteria that colonize mucosal surfaces.
Catalog #T0850-02
ApplicationsSuitable for use in Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Frozen sections only):1:25- 1:100.
ELISA: 1:5000- 1:20,000.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in Glycine Buffered Saline, pH 7.4, 0.1% sodium azide, 0.1% EACA, 0.01% Benzamidine, 1mM EDTA, before the addition of glycerol to 40%.
PurityPurified by ammonium sulfate precipitation.
ImmunogenNative purified human Tamm-Horsfall glycoprotein (uromucoid) from urine.
SpecificityRecognizes human Tamm-Horsfall Glycoprotein. No significant crossreactivity with urinary or other related human serum proteins. No known crossreactivity with other serum proteins in humans or other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links