You are here:Home
»
Antibodies
»
Abs to Carbohydrates, Glycoproteins
»
Anti -Tamm-Horsfall Glycoprotein (Uromucoid, THgp, Uromodulin, UMOD)
Anti -Tamm-Horsfall Glycoprotein (Uromucoid, THgp, Uromodulin, UMOD)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B |
|
| Tamm-Horsfall glycoprotein (THP, Uromodulin) is a 105-120kD urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619aa in length. It contains three EGF-like domains (aa28-148), a ZP domain that mediates oligomerization (aa335-590), and a cleavable C-terminal propeptide (aa619- 642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17aa substitution for aa601-642, and a 166aa substitution for aa441- 642. Over aa25-614, mouse Uromodulin shares 78% and 89%aa identical to human and rat Uromodulin, respectively. | | | | | | Applications: | | | Suitable for use in ELISA and Western Blot. Other applications not tested. | | | Catalog # | T0850-02B | | Recommended Dilutions | Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.3ng/well. | | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Mouse | | Concentration | ~0.2mg/ml | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. | | Purity | Purified by affinity chromatography. Endotoxin: <0.1 EU/ug (LAL) | | Immunogen | NS0-derived, recombinant mouse Tamm-Horsfall Glycoprotein (aa24-618, Accession #Q91X17). | | Specificity | Recognizes mouse Tamm-Horsfall Glycoprotein. Shows <5% crossreactivity with recombinant human Tamm-Horsfall Glycoprotein. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
