Anti -Tamm-Horsfall Glycoprotein (Uromucoid, THgp, THP, Uromodulin, UMOD) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B |
|
| Uromodulin (also Tamm-Horsfall glycoprotein or THP) is a 105-120kD urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619 amino acids (aa) in length. It contains three EGF-like domains (aa28-148), a ZP domain that mediates oligomerization (aa335-590), and a cleavable C-terminal propeptide (aa619-642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17aa substitution for aa601-642, and a 166 aa substitution for aa441-642. | | | Catalog # | T0850-05C | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant mouse Uromodulin is ~1ng/lane and 20ng/lane under non-reducing and reducing conditions, respectively. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Mouse | | Concentration | ~0.05mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, BSA (50ug per ug antibody). Labeled with Biotin. Reconstitute with 1ml sterile TBS, (20mM Trizma base, 150mM sodium chloride) pH 7.3, 0.1% BSA | | Purity | Purified by Immunoaffinity chromatography | | Immunogen | Recombinant corresponding to aa24-618 of mouse Uromodulin expressed in NSO cells. Accession# Q91X17. Species Sequence Homology | | Specificity | Recognizes mouse Uromodulin. Shows less than 5% cross-reactivity with recombinant human Uromodulin. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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