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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Tamm-Horsfall Glycoprotein (Uromucoid, THgp, THP, Uromodulin, UMOD) (Biotin)

Anti -Tamm-Horsfall Glycoprotein (Uromucoid, THgp, THP, Uromodulin, UMOD) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
Uromodulin (also Tamm-Horsfall glycoprotein or THP) is a 105-120kD urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619 amino acids (aa) in length. It contains three EGF-like domains (aa28-148), a ZP domain that mediates oligomerization (aa335-590), and a cleavable C-terminal propeptide (aa619-642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17aa substitution for aa601-642, and a 166 aa substitution for aa441-642.
Catalog #T0850-05C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1-0.2ug/ml. The detection limit for recombinant mouse Uromodulin is ~1ng/lane and 20ng/lane under non-reducing and reducing conditions, respectively.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceMouse
Concentration~0.05mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, BSA (50ug per ug antibody). Labeled with Biotin. Reconstitute with 1ml sterile TBS, (20mM Trizma base, 150mM sodium chloride) pH 7.3, 0.1% BSA
PurityPurified by Immunoaffinity chromatography
ImmunogenRecombinant corresponding to aa24-618 of mouse Uromodulin expressed in NSO cells. Accession# Q91X17. Species Sequence Homology
SpecificityRecognizes mouse Uromodulin. Shows less than 5% cross-reactivity with recombinant human Uromodulin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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