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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » TaqI

TaqI

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Specifications

5'-T^C G A-3'
3'-A G C^T-5'
Catalog #T1017
Concentration10u/ul
SourceThermus aquaticus YT-1
Supplied wtihR1625-80 Restriction Enzyme Buffer for TaqI, 10X: Supplied as a liquid in
10mM Tris-HCl pH 8.0, 5mM MgCl2, 100mM NaCl and 0.1mg/ml BSA. Use as 1x. Incubate at 65°C under paraffin oil in a capped vial. Incubation at 37ºC results in 10% activity.
R1625 Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9 at 37ºC.
Diluent Buffer10mM Tris-HCl pH 7.4,100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods–the Storage Buffer should be used.
Storage Buffer10mM Tris-HCl pH 7.5, 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol.
Compatible EndsBsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, Ssil, XmiI
Unit DefinitionOne unit is defined as the amount of TaqI to digest 1ug of lambda DNA dam- in 1 hour at 65°C in 50ul of assay buffer.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Thermal Inactivation
Enzyme is not inactivated by incubation at 80°C for 20min.
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA dam-x 16 hours) with TaqI.
Ligation/Recleavage Assay
The ligation and recleavage assay was replace with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of TaqI for 4 hours.
Methylation Effects
DamMay overlap- blocked
Dcm, EcoKI, EcoBlNever overlaps-no effect
CpGCompletely overlaps- no effect
Stability during Prolonged Incubation
A minimum of 0.3 units of TaqI is required for complete digestion of 1ug of lambda DNA in 16 hours at 65°C.
Number of Recognition Sites in DNA
Lambda121
PhiX17410
pBR3227
pUC574
pUC18/194
pTZ19R/U5
M13mp18/1912


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