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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Tau (Neurofibrillary Tangles Marker, Microtubule-associated Protein, MAP, Paired helical filament-tau, PHF-tau)

Anti -Tau (Neurofibrillary Tangles Marker, Microtubule-associated Protein, MAP, Paired
helical filament-tau, PHF-tau)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
Tau proteins are important in the assembly of microtubules. In normal brain, Tau is localized within the axons of the neurons, but in some neuropathological lesions it accumulates within the body of the neuron. Tau proteins are thought to be abnormally phosphorylated in certain disease states. Tau has been immunohistochemically localized to neurofibrillary tangles of Alzheimer’s disease (AD) brains. Tau proteins promote the assembly of tubulin monomers into microtubules and stabilize microtubules. Alternate splicing of Tau mRNA, glycosylation, and differential phosphorylation contribute to the heterogeneity of Tau.
Catalog #T1029
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml
Immunoprecipitation: 10ug/200-500ug of cell lysate
Immunohistochemistry (FFPE, frozen): 1-2ug/ml for 30-60 minutes at RT. For formalin-fixed paraffin embedded tissues, staining is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes prior to antibody incubation. Intensely stains the human neurofibrillary tangles, neuropil threads and neuritic plaques associated with Alzheimer’s disease. Observed to stain astrocytes.
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlHuman T98G glioblastoma, SH-SY5Y cells or brain tissue.
HybridomaSp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2B2.100
HostMouse
SourceBovine
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenPurified bovine microtubule-associated proteins.
SpecificityRecognizes and is highly specific for non-phosphorylated and phosphorylated forms of bovine Tau proteins (45-68kD). Does not crossreact with other microtubule associated proteins (MAPs) or tubulin. The epitope is located in the middle region of Tau.
Species CrossreactivityHuman, sheep, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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