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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Tau (Tau Protein, Microtubule-associated Protein, MAP)

Anti -Tau (Tau Protein, Microtubule-associated Protein, MAP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IF
Tau is a type II microtubule associated protein or MAP. Alternative splicing gives rise to 4–5 different forms of the tau protein (55–62kD). Tau is located in the axons and dendrites of nerve cells where it accelerates tubulin polymerization and crosslinks microtubule fibers into stabilized bundles promoting axon growth. It can also induce axon-like projections when expressed at high level in non-neuronal cells. All 6 tau isoforms of the CNS are expressed mainly in axons of the normal brain, but to a lesser extent tau may also be found in astrocytes. A 7th isoform, ‘Big Tau’, is expressed in peripheral nervous system. Tau is altered in Alzheimer’s disease to form paired helical filaments in neurofibrillary tangles, neurofilament threads and senile plaque neurites.
Catalog #T1030-02
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 5-10ug/ml
ELISA: 0.1-0.5ug/ml
Western Blot: 0.5-2ug/ml
Immunoprecipitation: 2-5ug per reaction
Immunohistochemistry (Frozen section, alcohol-fixed paraffin-embedded section and Bouin’s fixed): 5-10ug/ml; For alcohol-fixed paraffin-embedded section, antigen retrieval is not necessary.
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
SourceAscites
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3G287
HostMouse
SourceBovine
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.05% sodium azide before the addition of glycerol to 40%.
PurityPurified by Protein A affinity chromatography.
ImmunogenBovine Tau.
SpecificityRecognizes bovine Tau (binding-competent and binding-incompetent Tau) cytoskeletal protein and with A68 (an abnormal phosphorylated derivative of Tau). Recognizes aa141-178 of human Tau. Reactivity is not dependent on the phosphorylation state of Tau. Species Crossreactivity: Human. Does not crossreact with rat or mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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