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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Tau (Tau Protein, Microtubule-associated Protein, MAP)

Anti -Tau (Tau Protein, Microtubule-associated Protein, MAP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IH
Catalog #T1030-03
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoblot Analysis: A 1:500-1:2000 dilution of this lot detected Tau in human Alzheimer brain RIPA lysates. Human Alzheimer brain lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Tau (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunohistochemistry: An independent laboratory reported this lot detected Tau in paraffin-embedded human Alzheimer diseased brain sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No5H68
HostMouse
SourceBovine
ConcentrationNot determined
FormSupplied as a liquid with 0.05% sodium azide and 30% glycerol.
PurityAscites
ImmunogenNative bovine Tau. Epitope mapped to the C-terminus (aa 315-352).
SpecificityRecognizes Tau in several modified forms, Mr 50-60kD. Predicted to crossreact with MAP2 at 280kD. Species Crossreactivity: Human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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