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You are here:Home » Antibodies » Antibodies-Microtubule-associated Proteins » Anti -Tau (Tau Protein, Microtubule-associated Protein, MAP)

Anti -Tau (Tau Protein, Microtubule-associated Protein, MAP)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Tau protein is named for its ability to serve as a bridge for microtubule formation and stabilization. Tau in PNS neurons is 758 amino acids (aa) in length and 110 kD in size. It migrates anomalously in SDS-page. Its N-terminus binds plasma membrane components while its C-terminus binds microtubules. The microtubule-binding region contains four consecutive Tau/MAP repeats between aa 561-685. Each repeat is 30 aa long, Ser/Thr rich, and shows a PGGG motif. Multiple isoforms exist, ranging from 316 to 776 aa in length. Various deletions occur within the first 500 aa, with some isoforms also showing an absence of the second Tau repeat (aa 592-622). Between aa 624-756, human Tau protein shows absolute identity to mouse and dog Tau.
Catalog #T1030-04
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.5-1ug/ml. Detection limit is ~3ng/well.
Western Blot: 1-2ug/ml. Detection limit is ~5ng/lane under reducing and non-reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No7H166
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived recombinant human Tau (aa624-756) Accession # P10636
SpecificityRecognizes human Tau.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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