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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Tau, phosphorylated (Ser235) (Tau Protein, Microtubule-associated Protein, MAP)

Anti -Tau, phosphorylated (Ser235) (Tau Protein, Microtubule-associated Protein, MAP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Tau is a microtubule-associated phosphoprotein (MAP) localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms ranging in size from 352 to 441 amino acids. The tau variants differ from each other in the presence or absence of either three or four repeat-regions near the carboxy-terminal and the presence or absence of one or two inserts near the amino-terminal. Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. Over thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3 transfection phosphorylates serines 199, 202, 235, 396, 404, and 413 and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of Tau. Phosphorylation on these sites reduces the ability of a given tau species to promote microtubule self-assembly. Hyperphosphorylated tau is the major protein found in the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer’s disease (AD). These PHFs are also found in the lesions of other central nervous system disorders.
Catalog #T1030-06H1
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.05-0.2ug/ml
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS, PBS, pH 7.4, 0.02% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phospho-peptide (KLH). corresponding to the residues surrounding phospho-serine 235 of human tau.
SpecificityRecognizes endogenous levels of tau protein only when phosphorylated at serine 235.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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