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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Tau, phosphorylated (Ser199, Ser202) (Tau Protein, Microtubule-associated Protein, MAP)

Anti -Tau, phosphorylated (Ser199, Ser202) (Tau Protein, Microtubule-associated Protein,
MAP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of tau is to promote tubulin polymerization and stabilize microtubules. Tau, in its hyper-phosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimerís disease (AD) brain. Hyper-phosphorylated tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyper-phosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK-3b, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II, hosphorylate tau. Serines 199 and 202 are phosphorylated by GSK-3b and have been linked to hereditary frontotemporal dementia. Serine 202 phosphorylation by cdk5, stimulated by the presence of microtubules, has been linked to hereditary neurodegenerative disease.
Catalog #T1032-50
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilution to be determined by the researcher.
Positive ControlRecombinant human Tau or African green monkey kidney (CV-1) cells, stably expressing human four repeat tau and SV40 small T antigen to induce specific inhibition of PP2A.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol. Also available without BSA & Azide. See T1032-50A.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to human tau that contains serines 199 and 202. Species sequence homology: mouse and rat.
SpecificityRecognizes human Tau when phosphorylated when Ser199 and Ser202. Species sequence homology: Other species (100%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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