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Anti -Tau, phosphorylated (Ser199, Ser202) (Tau Protein, Microtubule-associated Protein, MAP) (BSA & Azide Free)
Anti -Tau, phosphorylated (Ser199, Ser202) (Tau Protein, Microtubule-associated Protein,
MAP) (BSA & Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of tau is to promote tubulin polymerization and stabilize microtubules. Tau, in its hyper-phosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer’s disease (AD) brain. Hyper-phosphorylated tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyper-phosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK-3 , protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II, hosphorylate tau. Serines 199 and 202 are phosphorylated by GSK-3 and have been linked to hereditary frontotemporal dementia. Serine 202 phosphorylation by cdk5, stimulated by the presence of microtubules, has been linked to hereditary neurodegenerative disease. | | | Catalog # | T1032-50A | | Applications | Suitable for use in Western blotting applications. | | Recommended Dilution | Western blot: 1:1000. The optimal antibody concentration should be determined empirically for each specific application. | | Positive Control Used | Recombinant human Tau or African green monkey kidney (CV-1) cells, stably expressing human four repeat tau and SV40 small T antigen to induce specific inhibition of PP2A. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Form | Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.2. No stabilizing proteins added. No preservative added. Glycerol-free. Also available with BSA & Azide. See T1032-50. | | Purity | Purified from rabbit serum by sequential epitope-specific chromatography. Negatively preadsorbed using a non-phosphopeptide corresponding to the sites of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide that is phosphorylated at serines 199 and 202. | | Immunogen | Synthetic phosphopeptide derived from the region of human tau that contains serines 199 and 202. The sequence is conserved in mouse and rat. | | Specificity | Recognizes Human Tau when phosphorylated at Ser199 and Ser202. Other species (100% homologous) have not been tested, but are expected to react. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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