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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Tau, phosphorylated (Ser199, Ser202) (Tau Protein, Microtubule-associated Protein, MAP) (BSA & Azide Free)

Anti -Tau, phosphorylated (Ser199, Ser202) (Tau Protein, Microtubule-associated Protein,
MAP) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of tau is to promote tubulin polymerization and stabilize microtubules. Tau, in its hyper-phosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer’s disease (AD) brain. Hyper-phosphorylated tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyper-phosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK-3 , protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II, hosphorylate tau. Serines 199 and 202 are phosphorylated by GSK-3 and have been linked to hereditary frontotemporal dementia. Serine 202 phosphorylation by cdk5, stimulated by the presence of microtubules, has been linked to hereditary neurodegenerative disease.
Catalog #T1032-50A
ApplicationsSuitable for use in Western blotting applications.
Recommended DilutionWestern blot: 1:1000. The optimal antibody concentration should be determined empirically for each specific application.
Positive Control Used Recombinant human Tau or African green monkey kidney (CV-1) cells, stably expressing human four repeat tau and SV40 small T antigen to induce specific inhibition of PP2A.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.2. No stabilizing proteins added. No preservative added. Glycerol-free. Also available with BSA & Azide. See T1032-50.
PurityPurified from rabbit serum by sequential epitope-specific chromatography. Negatively preadsorbed using a non-phosphopeptide corresponding to the sites of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide that is phosphorylated at serines 199 and 202.
ImmunogenSynthetic phosphopeptide derived from the region of human tau that contains serines 199 and 202. The sequence is conserved in mouse and rat.
SpecificityRecognizes Human Tau when phosphorylated at Ser199 and Ser202. Other species (100% homologous) have not been tested, but are expected to react.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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