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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Tau, phosphorylated (Ser199) (Tau Protein, Microtubule-associated Protein, MAP)

Anti -Tau, phosphorylated (Ser199) (Tau Protein, Microtubule-associated Protein, MAP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Tau is a neuronal microtubule-associated protein found predominantly on axons that functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer’s disease (AD) brain. Hyperphosphorylated tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases including GSK-3 , protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II, phosphorylate tau. Phosphorylation of serine 199 is catalyzed by GSK-3 in vitro and has been linked to frontotemporal dementia.
Catalog #T1032-51
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilution to be determined by the researcher.
Positive ControlRecombinant human Tau treated with GSK-3 ; African green monkey kidney (CV-1) cells, stably expressing human four repeat tau and SV40 small T antigen to induce specific inhibition of PP2A.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human tau that contains serine 199. Species sequence homology: mouse and rat.
SpecificityRecognizes human Tau when phosphorylated at Ser199. Species sequence homology: mouse and rat (100%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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