You are here:Home
»
Antibodies
»
Abs to Microtubule-associated Proteins
»
Anti -Tau, phosphorylated (Thr205) (Tau Protein, Microtubule-associated Protein, MAP)
Anti -Tau, phosphorylated (Thr205) (Tau Protein, Microtubule-associated Protein, MAP)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH |
|
| Tau is a neuronal microtubule-associated protein found predominantly on axons that functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer’s disease (AD) brain. Hyperphosphorylated tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases including GSK-3b, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II, phosphorylate tau. Microtubule-dependent phosphorylation of threonine 205 is catalyzed by GSK-3b and cdk5 in vitro. | | | Catalog # | T1032-55 | | Applications | Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Positive Control | Recombinant human Tau treated with GSK-3b | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | ~0.2 mg/ml | | Form | Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol. Also available without BSA, Azide. See T1032-55A. | | Purity | Purified by immunoaffinity chromatography | | Immunogen | Synthetic phosphopeptide derived from the region of human tau that contains threonine 205. The sequence is conserved in mouse and rat. | | Specificity | Recognizes human Tau when phosphorylated at Thr205. Species sequence homology: Mouse and rat (100%) . . | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
