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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Tau, phosphorylated (Thr212) (Tau Protein, Microtubule-associated Protein, MAP)

Anti -Tau, phosphorylated (Thr212) (Tau Protein, Microtubule-associated Protein, MAP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of tau is to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer’s disease (AD) brain. Hyperphosphorylated tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous threonine/threonine kinases, including GSK-3b, protein kinase A (PKA), cyclin-dependent kinase 5 and casein kinase II, phosphorylate tau. Threonine 212 is phosphorylated by GSK-3b, cdk5 and PKA in vitro and in vivo and has been shown to be specific for processes found in neurodegenerative diseases.
Catalog #T1032-56
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilution to be determined by the researcher.
Positive ControlRecombinant human tau left untreated, or treated with GSK-3b.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human tau that contains threonine 212. Species sequence homology: many species including mouse, rat, rhesus monkey, baboon, cow, and goat.
SpecificityRecognizes human Tau when phosphorylated at Thr212. Species sequence homology: many species including mouse, rat, rhesus monkey, baboon, cow, and goat (100%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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