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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Tau1 (Tau Protein1, Microtubule-associated Protein, MAP)

Anti -Tau1 (Tau Protein1, Microtubule-associated Protein, MAP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH
Catalog #T1037
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 5ng/ml. Bovine brain microtubule proteins purified by two cycles of assembly and disassembly (9) are dissolved in SDS-PAGE sample buffer. 5ug of the microtuble preparation per lane is loaded onto a 4% to 20% SDS-PAGE gradient gel along side molecular weight markers (14.3-200kD). After separation by electrophoresis, the proteins are blotted onto nitrocellulose. Tau is detected as a series of 5 bands (52–68kD).
Immunohistochemistry: 5ug/ml. Stains axons in tissue primarily, however in culture Tau expression is not restricted to just axons. Dephosphorylation with alkaline phosphatase is recommended for staining neurofibrillary tangles in Alzheimer’s brain tissue with T1037. This treatment changes the staining pattern of T1037 to include cell bodies, dendrites and axons of neurons. In untreated samples, T1037 stains axons only.
Optimal dilutions to be determined by the researcher.
Positive ControlIMR5 cells, Human T98G glioblastoma cells or Alzheimer’s disease brain tissue
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q123
HostMouse
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4.
PurityPurified immunoglobulin
ImmunogenPurified denatured bovine microtubule associated proteins
SpecificityRecognizes tau in bovine brain (SDS-PAGE). Species Crossreactivity: Human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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