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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Tau (Tau Protein, Microtubule-associated Protein, MAP)

Anti -Tau (Tau Protein, Microtubule-associated Protein, MAP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IF
Tau proteins are microtubule associated proteins (MAPs). Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau.
Catalog #T1037-01
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation and Western Blot. Not suitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Native verified): 2ug/mg protein lysate Use Protein G
Western Blot: 1-2ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.
Positive ControlIMR5 cells, Human T98G glioblastoma cells or Alzheimer’s disease brain tissue
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F348
HostMouse
SourceBovine
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T1037-01X.
PurityPurified by Protein G affinity chromatography.
ImmunogenPuri ed bovine microtubule-associated proteins. Epitope
SpecificityRecognizes the nonphosphorylated and phosphorylated forms of bovine Tau. Intensely stains the human neurofibrillary tangles, neuropil threads and neuritic plaques associated with Alzheimer’s disease. Does not crossreact with other MAPs or tubulin.
Species CrossreactivityHuman, sheep, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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