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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -Tau (Neurofibrillary Tangles Marker, Microtubule-associated Protein, MAP, Paired helical filament-tau, PHF-tau)

Anti -Tau (Neurofibrillary Tangles Marker, Microtubule-associated Protein, MAP, Paired
helical filament-tau, PHF-tau)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IC FC
Microtubule Associated Proteins, or MAPS, bind to the tubulin subunits of microtubule structures and regulate their functional stability. In the cell MAPs bind to monomer and multimerized tubulin. MAP binding to multimerized tubulin further stabilizes the formation of higher order microtubulin structures. MAP binding to microtubule structures is mediated through phosphorylation through Microtubule Affinity Regulated Kinase (MARK). Phosphorylation releases MAPs bound to microtubules, destabilizing the structure, driving it toward disassembly. There are predominately two MAP types, I and II. Type II MAPs include MAP2, MAP4, and tau, and are found in nervous tissue. Six tau isoforms exist in brain tissue, and they are distinguished by their number of binding domains. Three isoforms have three binding domains and the other three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (allowing it to bind to the negatively-charged microtubule). The isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.
Catalog #T1037-01C
ApplicationsSuitable for use in Flow Cytometry, Western Blot, and Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:160
Western Blot: 1:5000-20000
Immunocytochemistry: 1:100-500
Optimal dilutions to be determined by the researcher.
Positive ControlHuman brain tissue lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9i222
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
PuritySupernatant
ImmunogenPhospho-synthetic-peptide corresponding to residues surrounding Ser622 of human Tau 37.
SpecificityRecognizes human Tau phospholyated and unphospholyated on Ser622. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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