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You are here:Home » Culture Media » Media-Insect » TC-100 Insect Medium w/ L-Glutamine, NaHCO3 (Powder)

TC-100 Insect Medium w/ L-Glutamine, NaHCO3 (Powder)

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Specifications

Catalog #T2000
AppearanceWhite to off white, homogenous, free flowing powder.
SolubilityColorless to light yellow, clear, complete.
pH6.5 ± 0.5
Directions per LiterDissolve 19.4 grams in 800-900 mL of distilled/deionized (DDI) water, stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Note: On occasion, this formulation seems reluctant to dissolve. If continuous stirring with warmth has not produced complete dissolution, add 1.0N HCl slowly until solution turns crystal clear with light yellowish tone (pH 4.2-4.5). Let solution cool to RT, then back titrate with 1.0N NaOH to pH 6.2-6.4. Components will remain solubilized.
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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